Abstract
Amylase is one of the important digestive enzymes involved in hydrolysis of starch. In this paper, we describe a novel approach to study the interaction of amylase enzyme with gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) and checked its catalytic function. AuNPs are synthesized using citrate reduction method and AgNPs were synthesized using biological route employing Ficus benghalensis and Ficus religiosa leaf extract as a reducing and stabilizing agent to reduce silver nitrate to silver atoms. A modulatory effect of nanoparticles on amylase activity was observed. Gold nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. Immobilized amylase showed 1- to 2-fold increase of activity compared to free enzyme. The biocatalytic activity of amylase in the bioconjugate was marginally enhanced relative to the free enzyme in solution. The bioconjugate material also showed significantly enhanced pH and temperature stability. The results indicate that the present study paves way for the modulator degradation of starch by the enzyme with AuNPs and biogenic AgNPs, which is a promising application in the medical and food industry.
Highlights
Nanoparticles are defined as particulate dispersions with a size in the range of 10–100 nm [1]
The nanostructures have a great potential in biotechnological processes taking into account that each may be used as carriers for enzymes during different biocatalytic transformations [2]
The present study reveals the modulatory effect of AuNPs and biosynthesized AgNPs on enzyme activity during the hydrolysis of starch
Summary
Nanoparticles are defined as particulate dispersions with a size in the range of 10–100 nm [1]. Interaction of metal nanoparticles with biomolecules has received much attention in the recent years for the development of diagnostics, for sensors, and for targeted drug delivery. Interaction with AuNPs has been found to enhance the stability of peptides [17] Their unique property arises from higher surface to volume ratio and increased percentage of atoms at the grain boundaries. They represent an important class of materials in the development of novel devices that can be used in various physical, biological, biomedical, and pharmaceutical applications [18,19,20]. Synthesized silver or gold nanoparticles help to produce new insecticides and insect repellants
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