Modulation of immune response by varying ligand density on the surface of nanoparticles (42.22)

Abstract

Abstract Targeting antigen presenting cells with biodegradable nanoparticles provides an excellent opportunity for the development of vaccines. Polymeric nanoparticles with entrapped antigens serve as an excellent platform for controlled release of antigens. Immune response can be optimized and enhanced by selectively targeting the antigen loaded nanoparticles to dendritic cells. Here we report our findings using the PLGA nanoparticulate system to target DCs by targeting their surface receptor DEC205. These nanoparticles encapsulate the model antigen ovalbumin and are surface modified with anti DEC205 at different ligand densities. Our studies demonstrate that targeting bone marrow derived DCs using different modifications of nanoparticles but targeting the same receptor lead to altered T cell responses. These studies have implications in designing a novel nanoparticle based vaccine delivery system.