Abstract
BackgroundIn this paper, we present data on the influence of low level magnetic fields (0.5 μT‐2 μT) on the modulation of hydrogen peroxide (H2O2) biogenesis in human fibrosarcoma cancer cell line HT1080, and bovine pulmonary artery endothelial cells (PAEC) compared to cells exposed to geomagnetic field (control; 45 μT‐60 μT).MethodsThe extracellular quantification of the production of H2O2 may be a direct measure of the production and decomposition of superoxide radical. H2O2 can freely pass through cell membranes and can be detected outside the cell. For the quantification of H2O2 we used a method that has been successfully applied before. This method involves the oxidation of Amplex Red by H2O2 in the presence of horseradish peroxidase (HRP). Tri‐axial Helmholtz coils established the control static magnetic field. Low level fields were implemented by a μ–metal cylinder. The background time‐varying magnetic field was measured by induction with a sensor comprised of a set of square perpendicular coils.ResultsReduction of the Earth's magnetic field suppressed H2O2 production in cancer cells and PAEC. The addition of catalase and superoxide dismutase (SOD) mimetic MnTBAP inhibited the magnetic field effect on H2O2 production.ConclusionBecause most cancer cells undergo increased oxidative stress and ROS production due to excessive metabolic requirements to support an increased rate of cell division, it is reasonable to consider investigating a therapeutic that might target cancer cells through a free radical mechanism. Whether radio frequency exposure can influence growth rates and H2O2 production remains open and we propose to investigate it in the future. Modulating ROS production by magnetic fields may open new venues of biomedical research and therapeutic strategies.
Published Version
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