Abstract
Patch clamp experiments with fixation of the membrane potential were performed on isolated rat prefrontal cortex neurons to study the modulation of ion currents induced by applications of GABA and kainate by GABAB receptors and group I metabotropic glutamate receptors. Blockade of GABAB receptors with the selective antagonist CGP-55845 (5 μM) increased the peak amplitude of the ion current induced by application of GABA (40 μM) by 26 ± 13% (n = 6). The amplitude of the ion current plateau at 14 sec of application of GABA was the same in controls as with blockade of GABAB receptors. The long-term effects of activation of GABAB receptors were studied in terms of the responses to application of GABA and kainate in the presence of baclofen (50 μM), a selective GABAB receptor agonist. Prolonged prior application of baclofen increased the amplitude of responses to application of GABA by 9 ± 2% (n = 8) as compared with controls. Responses to application of kainate did not change in the presence of baclofen. Analogous experiments using trans-ACPD, a selective agonist of groups I and II metabotropic glutamate receptors, did not reveal any changes in responses to application of GABA or kainate. These data point to modulation of GABAA receptors by postsynaptic metabotropic GABAB receptors in rat cerebral cortex neurons
Published Version
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