Abstract
Vasoactive intestinal peptide stimulates phosphorylation of six high molecular weight cytosolic proteins in the cultured retinal pigment epithelium (RPE). Of these, the 190-kD phosphoprotein is associated with the microtubules assembled by taxol/GTP and is immunologically related to the brain microtubule-associated protein 2 (mol.wt. = 280 kD). VIP is also shown here to stimulate secretion in the cultured RPE. VIP-stimulated phosphorylation of a 190-kD microtubule-associated protein is also demonstrated here in the retinal glia.
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