MODULATION BY SODIUM INTAKE OF CARDIORENAL AND METABOLIC CHANGES INDUCED BY HIGH FRUCTOSE DIET IN RATS: PP.29.143

Abstract

High fructose (HF) diet is a well known model of insulin resistance in rats, associated with slightly increase in blood pressure, cardiac hypertrophy and renal damages. The aim of this study was to evaluate the influence of sodium intake on cardiorenal and metabolic changes associated with HF diet in Sprague-Dawley rats. At the age of 6 weeks, rats fed 4 types of diet for 12 weeks: normal salt control (NSC, 0.64% NaCl), normal salt-60% fructose (NSF), low salt-60% fructose (DSF, <0.01% NaCl), or high salt-60% fructose (HSF, 8% NaCl) diet. At the end of the diet, tail-cuff pressure (TCP) was measured. Glucose metabolism was assessed by intra-peritoneal glucose and insulin tolerance tests. Cardiac morphology and function were evaluated by echocardiography. Urinary albumin excretion (UAE) was measured by ELISA test. Heart weight index (HWI) was determined. Fructose diet induced insulin resistance, cardiac hypertrophy and albuminuria, and these damages were amplified by high sodium intake independently of blood pressure. Low sodium intake prevented albuminuria without change of blood pressure but had no significant effect on cardiac hypertrophy. Sodium intake did not influence insulin resistance associated with high fructose diet. Effects of sodium intake on cardiac and renal changes induced by high fructose diet were independent of blood pressure and glucose metabolism. The role of oxidative stress should be assessed.