Modified nutrient medium for isolation and identification of non-fermenting bacteria

Abstract

The relevance of this study is due to the need for laboratory monitoring of the circulation of gram-negative non-fermenting bacteria that have a high etiological significance in the occurrence of nosocomial infections, as well as the complexity of differentiating these bacteria on simple nutrient media from representatives of enterobacteria and between individual representatives of gram-negative non-fermenting bacteria.
 The purpose of this study was to design a differential diagnostic medium containing domestic ingredients.
 Materials and methods. The growth efficiency of test strains on comparable nutrient media, their cultural and morphological characteristics were determined.
 Results. The modified culture medium makes it possible to distinguish test strains of gram-negative non-fermenting bacteria from test strains of other bacteria (gram-negative enterobacteria, Staphylococcus), and between these strains by the color of the colonies and growth patterns. This feature gives the opportunity to apply modified medium for the differentiation of non-fermentative bacteria from fermenting ones and, simultaneously, for the primary differentiation of different representatives of non-fermenting bacteria by changing the color of the medium and different color of colonies.