Modified method of producing sciatic nerve crush injury model in Wistar rats: A pilot study

Abstract

The production of a standard and reproducible experimental mode of peripheral nerve injury, has been a long-time quest in nerve regeneration research. The aim of this pilot study was to reproduce sciatic nerve crush injury model in Wistar rats and also use the established method for broader and future research. Materials and Methods: twenty (20) rats grouped into five (5) of five (5) rats each. Normal control (G1), the sciatic was not exposed nor crushed; Sham (G2), the sciatic nerve were surgically exposed but not crushed; Crushed (G3), the sciatic nerve were surgically exposed and uniformly crushed, using a non-serrated heamostatic forceps that exerted a compressive force of 33N for a duration of 30 seconds and Transected (G4), the sciatic nerve were exposed and completely transected. Sciatic nerves were harvested at seven (7) days (post-injury) for histological evaluation and the groups were compared for histopathological changes. Results: Transverse sections of toluidine blue (TB) stained sciatic nerve micrograph, showed degenerative changes in the axons, myelin ballooning and surveiling white blood cells, all of which were consistent with Wallerian degeneration in G3, when compared with groups G1 and G2. The G4 group showed more severe degenerative changes when compared with group G3. Conclusion: The histopathological analysis suggest that the modified induction method used in this study, caused Axonotmetic lesion in the crushed sciatic nerves of the Wistar rats.