Abstract
A modification of a gas—liquid chromatographic method is described that allows better simultaneous separations of the neutral and aminosugar alditol acetate derivatives as single peaks. Using 3% SP-2340 on 100–200 mesh Supelcoport, retention times were relatively short and baseline separation between glucose and galactose was achieved. The method is particularly suitable for monitoring the fractionation of complex mixtures of glycoproteins and glycosaminoglycans, and its application is illustrated in the fractionation of bone matrix extracts subjected to ion-exchange chromatography. A convenient procedure allowing the separation and estimation of sialic acid in the same aliquot is also described and evaluated.
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