Abstract
The article presents the results of studies on modification of the phage titer increase reaction technique for aeromonad bacteriophages. Using bacteriophages Av-4 of the UlSAU series and Ah-4 of the Ul-SAU series, which are characterized by high titers of lytic activity and a wide spectrum of specific lyt-ic action, the optimal parameters for setting up the phage titer increase reaction (a quantitative indicator of the reaction that has diagnostic value and the optimal time that ensures full interaction of the phage with bacteria) were developed. It was empirically established that the following parameters are the most effective for setting up the RNF for the purpose of indicating A. veronii bv.veronii and A.hydrophila bacteria in sanitary inspection facilities: the concentration of the indicator culture de-tected during the reaction is 103 - 104 m.c./ml/g, the working dilution of the bacteriophage is 105 PFU/ml. Experiments to optimize the methodology for setting up the RNF have shown that the most optimal method for detecting A. hydrophila and A. veronii bv. veronii bacteria in non-sterile water and fish samples in laboratory conditions is preliminary cultivation of the test material for 2 hours at (29±1) 0C, followed by infection with phages Av-4 of the UlSAU series and Ah-4 of the UlSAU series and incubation of the mixture for 4 hours at (29±1) 0C. The study was carried out in accordance with the thematic plan-task of the Ministry of Agriculture of the Russian Federation, registration number EGISU NIOKTR 123031600041-9.
Published Version
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