Abstract
The aim of the study was to modify a simple and widely used spectrophotometric assay for MAO activity evaluation with 2,4-dinitrophenylhydrazine. A modified procedure includes molar absorption coefficients of 2,4-DNP-hydrazone benzaldehyde and 2,4-DNP-hydrazone 5-hydroxyindolylacetaldehyde as 2.3 × 104mol−1l cm−1 and 1.0 × 104 mol−1l cm−1, respectively. Such an approach allows to express specific enzyme activity as nmol product formed/min/mg protein.
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