MODERN MICROBIOLOGICAL TECHNOLOGIES IN DIAGNOSIS OF MYCOSES IN OTORHINOLARYNGOLOGICAL PRACTICE

Abstract

The review article highlights the latest approaches to solve one of the important problems of modern otorhinolaryngology, the diagnosis of opportunistic mycoses of the upper respiratory tract and ear. Opportunistic mycoses of the ENT organs in recent decades have posed a significant challenge for modern clinical medicine not only in Ukraine but also throughout the world. According to the scientific literature, the share of fungal lesions of the ear and upper respiratory tract in the structure of chronic inflammation of these organs makes up 22.1%. The main causative agents of mycotic lesions of the ENT organs are opportunistic fungi of the genera Aspergillus, Рenicillium, Mucor and yeast-like fungi of the genus Сandida, which are characterized by a low level of pathogenicity and are a part of the resident microflora of the macroorganism. Classical methods of microbiological diagnosis have certain limitations in the identification of micromycetes. Therefore, the use of more reliable, fast and accurate methods in clinical practice will contribute to the timely and effective treatment of fungal diseases of the ENT organs. This review presents an analysis of modern microbiological diagnostic technologies, such as biochemical detection of microorganisms using identification plate test systems (ARI RapID, CrystalTM), semi-automatic and automatic microbiological identification using analyzers VITEK, VITEK 2, Walk Away. Identification of fungal pathogens can also be performed by performing direct protein profiling using mass spectrometry, polymerase chain reaction and sequencing. These methods with the highest level of reliability enable to identify the pathogen, as well as to assess its sensitivity to chemotherapeutic drugs. The combination of classical and modern microbiological technologies should become a standard for the diagnosis of fungal diseases, including the upper respiratory tract and ear.