Abstract
Hierarchical phosphorylation of the disordered 4E-BP2 protein stabilizes a binding incompatible 4-stranded beta domain while the C-terminal domain remains disordered. Ensemble descriptions of both phosphorylation states were calculated. The ensembles were restrained using Small-angle X-ray scattering (SAXS) and Paramagnetic Resonance Enhancement (PRE), while the single-molecule Förster Resonance Energy Transfer (smFRET) between residues 32 and 91 was used as validation of the ability of the restrained ensemble to agree with independent experimental evidence.
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