Abstract
Hierarchical phosphorylation of the disordered 4E-BP2 protein stabilizes a binding incompatible 4-stranded beta domain while the C-terminal domain remains disordered. Ensemble descriptions of both phosphorylation states were calculated. The ensembles were restrained using Small-angle X-ray scattering (SAXS) and Paramagnetic Resonance Enhancement (PRE), while the single-molecule Förster Resonance Energy Transfer (smFRET) between residues 32 and 91 was used as validation of the ability of the restrained ensemble to agree with independent experimental evidence.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
