Modeling of the Purulent-Inflammatory Process in Mice Against the Background of Immunosuppression Using Hydrocortisone and 2, 6, 10, 14-tetramethylpentadecane. In Vivo Research

Abstract

Purulent complications of wounds of various etiologies are one of the pressing problems of modern medicine. Antibiotic resistance is currently giving impetus to the development of ever newer and more advanced drugs, or the modernization of forms and delivery methods of existing ones. The purpose of the study was to reproduce the purulent wound model in mice and compare immunosuppression methods using hydrocortisone and the drug 2, 6, 10, 14-tetramethylpentadecane. Methods. Several variants of modeling the development of a purulent-inflammatory process in skin wounds in mice and rats were performed. When conducting an experiment on mice, three groups were divided: 1. Using hydrocortisone as immunosuppression (at the rate of 25 mg/kg for 7 days). Wounds were applied on the second day of drug administration. Based on the results of 14 individuals. 2. Using the drug Pristane as immunosuppression (at the rate of 500 μl intraperitoneally per 1 individual, 1 time). The wounds were applied on the 7th day. Based on the results of 14 individuals. 3. 14 mice were used in the control group - without immunosuppression. Then two types of bacteria were tested as wound-infecting microorganisms: Staphylococcus aureus, a representative of the normal skin microbiota, and Pseudomonas aeruginosa, as the most common type of pseudomonas, causative agents of nosocomial infections. Wound infection was carried out using a mixed suspension of the above 2 bacterial cultures. Results. We determined the most optimal model of purulent wounds, namely, the variant with the use of immunosuppression with 2, 6, 10, 14-tetramethyl-pentadecane. The use of this preparation allowed to reduce the number of immunosuppress or injections and to obtain a denser biofilm on the wound surface. Conclusion. Modeling of purulent wound in mice is possible only on the background of immunosuppression, as which can be used preparations 2, 6, 10, 14-tetramethyl-pentadecane and hydrocortisone.