Modeling of ayurveda ghee based solid lipid nano particles and their comprehensive pharmacokinetics study

Abstract

Several experiments have been done with solid lipid nanoparticles (SLNPs) module to produce a potential oral drug delivery system. However, the fate of the drug after its delivery in gastro-intestinal tract directly rely on the bioavailability. This study is focused on the problem concerned with the pre-prediction accuracies of the SLNPs formulation using in silico modeling and also an in vitro model for the synthesis of an efficient SLNPs carnitine complex. Carnitine is a molecule that helps in β- oxidation of fatty acids. Structure of carnitine and its nano complexes were modeled using Chemsketch tool. The pK/pB evaluation of these complexes was studied using ADMET predictor and GastroPlus tools. Further experiments were carried out with optimised ratio based on the drug:lipid ratio and surfactant: co-surfactant ratio. The mean particle size was measured to be 559.4 nm and higher density of particles size ranges between 68 nm and 458.7 nm. The entrapment efficiency of the SLNPs observed to be 65% and carnitine loading capacity was determined as 50%. The study of carnitine release kinetics by in vitro and ex vivo method was proved that the formulation possessed a sustained release of the carnitine with better bio-availability via oral delivery.