Abstract
Based on graphene sheet (GS) and gold-magnetic nanoparticles (Au–Fe3O4), a novel bioelectrochemical sensor was developed to detect toxoplasmosis-specific IgM antibody (Tg-IgM). This kind of bioelectrochemical sensor combined the high specific surface area of GS and the high biocompatibility of Au–Fe3O4 nanomaterials. The anti-IgM-HRP modified by Au–Fe3O4 nanoparticles was used as the detection probe, and the preparation method of composite detection probe and the corresponding sensor performance were provided. In addition, by a systematic simulation modeling, this study further investigated the effect of CV scanning speed, pH value, H2O2 concentration and graphene concentration on the detection sensitivity of the proposed bioelectrochemical sensor.
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