Abstract
To evaluate and compare the mycobacterial load using a mobile laminar airflow (LAF) device in an IVI-dedicated outpatient clean room (OCR) without ventilation systems and in a hospital-based operating theatre (HOT). This case-control study was conducted in 2 different settings: OCR and HOT during a series of intravitreal injections (IVIs). The Air Microbial analysis was performed using a Surface Air System instrument at three different moments during the IVI sessions in both settings: at the operative site (OS), four meters from the OS (DOS) and in the disinfection room (DR). At the OS in the OCR, the microbial load was 20.33 ± 16.51CFU/m3 at T-1; 1.00 ± 1.00CFU/m3 at T0; 6.67 ± 6.03CFU/m3 at T1; 10.00 ± 9.54CFU/m3 at T2 and 4.33 ± 3.06CFU/m3 at T3. At the OS in the HOT, the CFU were respectively 0.00 ± 0.00 at T0; 0.67 ± 0.58 at T1; 0.00 ± 0.00 at T2 and 0.00 ± 0.00 T3. This standardized sterile technique in an OCR setting, with a mobile LAF, reduces the microbial colonies to levels that are safe and comparable to those recorded in the HOT setting, thus potentially improving the safety of IVIs in an OCR setting.
Published Version
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