Abstract
Sequence analysis of ∼25 kb of an Azospirillum brasilense Sp245 85-MDa (∼142 kb) plasmid, p85, identified two novel IS elements mediating p85 fusions with a suicide plasmid vector, pJFF350. These IS elements, 1465-bp IS Azba1 and 1112-bp IS Azba3, belong to the IS 256 family and to the IS 5 family/IS 903 group, respectively. Truncated IS Azba2 from the ISL 3 family was found near one of the copies of IS Azba1 that flank pJFF350 in p85::pJFF350. As another factor potentially contributing to the known genetic plasticity of p85, a phage integrase gene was identified in this plasmid.
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