Abstract
Campylobacter jejuni infection is one of the most frequently reported foodborne bacterial diseases worldwide. The main transmission route of these microorganisms to humans is consumption of contaminated food, especially of chicken origin. The aim of this study was to analyze the genetic relatedness of C. jejuni from chicken sources (feces, carcasses, and meat) and from humans with diarrhea as well as to subtype the isolates to gain better insight into their population structure present in Poland. C. jejuni were genotyped using multilocus sequence typing (MLST) and sequence types (STs) were assigned in the MLST database. Among 602 isolates tested, a total of 121 different STs, including 70 (57.9%) unique to the isolates' origin, and 32 STs that were not present in the MLST database were identified. The most prevalent STs were ST464 and ST257, with 58 (9.6%) and 52 (8.6%) C. jejuni isolates, respectively. Isolates with some STs (464, 6411, 257, 50) were shown to be common in chickens, whereas others (e.g. ST21 and ST572) were more often identified among human C. jejuni. It was shown that of 47 human sequence types, 26 STs (106 isolates), 23 STs (102 isolates), and 29 STs (100 isolates) were also identified in chicken feces, meat, and carcasses, respectively. These results, together with the high and similar proportional similarity indexes (PSI) calculated for C. jejuni isolated from patients and chickens, may suggest that human campylobacteriosis was associated with contaminated chicken meat or meat products or other kinds of food cross-contaminated with campylobacters of chicken origin. The frequency of various sequence types identified in the present study generally reflects of the prevalence of STs in other countries which may suggest that C. jejuni with some STs have a global distribution, while other genotypes may be more restricted to certain countries.
Highlights
Campylobacter jejuni is one of the most frequently reported causes of foodborne bacterial enteric diseases worldwide, with a total of 246,307 laboratory confirmed infections in the European Union in 2017 [1]
The objectives of the study were: (i) to analyze the genetic relatedness of C. jejuni isolates obtained from chicken sources and from patients suffering from campylobacteriosis and (ii) to subtype the isolates to gain better insight into the population structure of C. jejuni recovered in Poland and to compare it with molecular variants identified in other countries
Most samples were isolated in 2016, in that year only 2 C. jejuni were collected from patients with campylobacteriosis
Summary
Campylobacter jejuni is one of the most frequently reported causes of foodborne bacterial enteric diseases worldwide, with a total of 246,307 laboratory confirmed infections (notification rate of 64.8 cases per 100,000 population) in the European Union in 2017 [1]. MLST typing of C. jejuni from different sources decision to publish, or preparation of the manuscript
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