Abstract

The isolated Messastrum gracile SVMIICT7 was mixotrophically cultivated in flat panel photobioreactor (FP-PBR) towards understanding the photosynthetic transient and product profile. Biomass productivity attained a maximum of 45 mg L-1d-1, with COD, nitrate and phosphate removal of 83.3%, 84.05%, and 74.98% respectively. Messastrum sp. showed good assimilation of proteins (124 mg g−1) (w/w), carbohydrates (119 mg g−1) (w/w) and lipids (26%) (w/w). The myristoleic acid (C14:1–39.1%) and heptadecanoic acid (C17:0–29.1%) are abundant fatty acids with therapeutic, food and feed applications. The cellular ultrastructure studies revealed facile arrangement of chloroplast and starch covered pyrenoids supporting increased carbohydrate accumulation. Photosystem II (PSII) [Y(II), ETR(II), Y(NPQ), and Y(NO)] and photosystem I (PSI) [Y(I), ETR(I), Y(NA), and Y(ND)] transients showed improved photosynthetic efficiency directing microalgae growth and biomass productivity. Higher Fv/Fm values indicates relatively good water splitting and carbon fixation at PSII and PSI facilitating improved photosynthetic electron transport and synthesis of value-added products thereby enabling bioeconomy.

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