Abstract
Objective: Evaluate, in vivo, the influence of mixing failures on endodontic sealers. Material and methods: To alveolus analysis, 80 rats were divided into Sealapex® and AH Plus® groups. Within each group, the sealer was subjected to either partial (incomplete homogenization—simulating handling failures) or total mixing (complete homogenization) over two periods of 7 and 30 days (n = 20). The maxillary incisor was extracted and a polyethylene tube containing the sealer was inserted. To quantify edema, 40 male rats were divided into four groups (n = 10). The animals received 2% Evans Blue intravenously, and either AH Plus® or Sealapex® was injected subcutaneously. The rats were euthanized after 3 or 6 hours and analyzed in a spectrophotometer (630 ?m). To analyze the subcutaneous tissue, 20 rats received polyethylene tube implants with the sealers in the dorsal area (n=10), then euthanized after either 7 or 30 days, and inflammation was evaluated according to an inflammatory cells score. Results: In the alveolar 7-day group, control group presented an inflammation score 1, while all other groups presented a score 2, except AH plus® total mix group (3). After 30 days, all groups presented a score 1. The edemogenic test showed less edema in Sealapex® groups (p < 0.5). In subcutaneous 7-day period, all groups presented score 2. In 30 days, all groups revealed score 1, except AH Plus® partial mix group (2). Conclusion: Regarding mixing of the sealers, there were no significant differences among the groups.KeywordsAH Plus; Inflammation; Materials testing; Root canal filling materials; Sealapex.
Highlights
A mong the numerous materials used in endodontic therapy, dentists should take special care with those used in obturation, because they are inserted into the root canals and remain in close contact with the apical tissues [1], filling irregularities in the root walls and accessory canals that gutta percha cannot penetrate [2]
The AH Plus® Total Mix showed a lack of fibroblasts in the connective tissue showed and no bone or blood vessel formation was observed
At 30 days, the inflammation was mild for all groups, with exception of AH Plus Total Mix®, which presented a decrease in the inflammatory infiltrate, but most of specimens achieved a moderate inflammation, without statistical significance (p > 0.05)
Summary
A mong the numerous materials used in endodontic therapy, dentists should take special care with those used in obturation, because they are inserted into the root canals and remain in close contact with the apical tissues [1], filling irregularities in the root walls and accessory canals that gutta percha cannot penetrate [2]. Sealapex® (SybronEndo, Glendora, CA) releases high amounts of calcium and hydroxyl ions [9,10,11]. It shows excellent tissue response [12] and has an antimicrobial effect against Enterococcus faecalis [13]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
