Mixed Micellization and Mixed Monolayer Formation of Sodium Cholate and Sodium Deoxycholate in Presence of Hydrophobic Salts Under Physiological Conditions

Abstract

AbstractMixed micellization and mixed monolayer formation of two bile salts namely sodium cholate (NaC) and sodium deoxycholate (NaDC), in the presence of sodium chloride (NaCl) and three hydrophobic salts including sodium acetate (NaAc), sodium butanoate (NaBu) and sodium hexanoate (NaHx) in 10 mM phosphate buffer (pH 6.5) at 37 °C were investigated by means of surface tension measurements. The experimental results were utilized to evaluate various parameters like critical micellar concentration (CMC), micellar and monolayer interaction parameter (β and βσ), micellar and monolayer mole fractions (X and Z), activity coefficients of two bile salts in mixed micelles and monolayer (f and f(σ)), surface excess (Γmax) and minimum surface area per molecule of bile salt (Amin). Mixed micelles and mixed monolayer were found to show slight non‐ideality and both these phenomena have been found to be affected differently in the presence of various additive salts with NaHx showing larger effects. Higher efficiency of NaHx in affecting both phenomena has been attributed to its appreciable hydrophobicity and surface activity, thus showing stronger interactions with bile salt molecules.