Mitotic effects of copper oxide nanoparticle on root development and root tip cells of Phaseolus vulgaris L. seeds.

Abstract

Copper oxide nanoparticle (CuO NP) is used widely in many fields in nanotechnology. For this reason, both production, use, and release to the environment are increasing with each passing day. With the increased use of products that contain nanoparticles (NP) (<100 nm), plants and organisms that constitute the food chain are at risk. In the present study, Phaseolus vulgaris L., a very common food plant, was exposed to metal-based CuO NPs. The anomalies that were caused by CuO NP in germination and mitosis of P. vulgaris were investigated. In the trials, a total of 4 groups (Control, 50, 150, and 300 ppm) were formed and examined in three replications. The determination of the accumulation and elimination rate because of NPs in P. vulgaris that was used in the study was made through X-ray diffraction (XRD), scanning electron microscope (SEM), mapping image, and EDX characteristic spectrum analysis. Also, the mitotic effects on germination, root development, and root tip cells of seeds that were grown by treatment with control, 50, 150, and 300 ppm concentrations were investigated. The study was conducted in three replications in a laboratory setting. All concentrations of CuO NPs caused significant decreases in the mitotic index in the root tip cells of P. vulgaris when compared to the control. The mitotic index reached the lowest level, especially at the highest concentration. Multiple analyzes in the study showed that CuO NPs cause abnormalities in cell division such as C-metaphase, distorted metaphase, distorted anaphase and telophase, chromosome breakage, asynchronous division, advanced chromosomes, micronucleus, and loss of genetic material. These findings also support that the Cytogenetic Test of P. vulgaris can be used to evaluate the genotoxicity of new nanomaterials that are used in many consumer products. In this respect, NPs that are taken up by the organisms in the food chain may pose a danger to higher consumer organisms when they accumulate in the tissue. A control mechanism must be established for the use and contamination of these particles and wider studies must be conducted regarding their effects. HIGHLIGHTS: The effects of CuO nanoparticle, which has a very wide usage area, on root development and mitosis of Phaseolus vulgaris L. plant were investigated in the study. The abnormalities of mitotic division on interphase, prophase, metaphase, anaphase, and telophase were visualized. Evaluation was made considering scanning electron microscopy (SEM) and X-ray diffraction (XRD) results as well.