Abstract
After addition of mitomycin C to Pseudomonas aeruginosa strain MAC-264 cells, DNA synthesis stops but resumes again after 45 min. This resumption in DNA synthesis is due to the induction of a temperate phage. Early after induction, both RNA and protein syntheses continue but later on little or no net synthesis of these macromolecules occurs. Cell lysis occurs at 90 to 105 min after addition of mitomycin C. Analysis of the lysates indicates that a DNA phage with an icosahedral head about 55 mμ in diameter with a short wedge-shaped tail is produced. This phage [Formula: see text] has a buoyant density of 1.468 g/ml in CsCl. The guanine + cytosine content of the phage DNA determined by its buoyant density in CsCl is 46% compared to 68% for the host DNA.Some phage tail-like structures which have a buoyant density of 1.305 g/ml in CsCl are also induced at the same time.
Published Version
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