Abstract
Abstract Peripheral blood lymphocytes from normal donors were incubated with mitogens in the presence or absence of exogenous DNA. The addition of DNA inhibited the response of the cells to the mitogens by up to 40% but did not alter the time course of the response. Similar inhibition was seen with the synthetic deoxyribonucleotides poly(dAdT) and poly(dA). Addition of the DNA up to 24 hr after the addition of mitogen to the cells did not affect the extent of inhibition. The inhibition was apparently due to a decrease in the total number of cells responding to the mitogen rather than to a general decrease in response of each cell. Furthermore, no specific cytotoxic effect of the exogenous DNA could be detected, and the degree of inhibition was approximately constant for different mitogen concentrations. In contrast, DNA alone induced a low degree of stimulation in the cells.
Published Version
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