Abstract
Objectives To study how thermal energy is converted after moxibustion at local skin from the view of mitochondrial respiratory chain and its key regulatory elements of sirtuins 1 (SIRT1) and sirtuins 3 (SIRT3). Methods Two moxibustion temperatures usually used in clinical practice (38°C and 46°C) were applied to Zusanli (ST36) acupoint for 30 minutes in C57BL/6J mice. Local skin samples were harvested at 30 min and 72 h after moxibustion intervention, respectively. The activity of mitochondrial respiratory chain complexes I–V was detected by spectrophotometry. The expression of SIRT1 and SIRT3 protein was detected by immunofluorescence staining or western blot. Results Moxibustion at 38°C triggered more significant increase of mitochondrial respiratory chain complexes I–V expression. However, the protein expression of SIRT1 and SIRT3 at 46°C showed more obvious enhancement. In addition, the effect of mitochondrial respiratory chain complexes I–V activity on local skin of ST36 acupoint was more obvious at 30 min after moxibustion, while the expression of SIRT1 and SIRT3 protein was more significant at 72 h after moxibustion. Conclusion Mitochondrial respiratory chain and its key regulatory element proteins SIRT1 and SIRT3 play important role in the initial process of thermal energy conversion stimulated by different moxibustion temperatures in local skin.
Highlights
Moxibustion is one of the conventional thermal therapies in traditional Chinese medicine, which has been widely used in clinical practice to treat various disorders in China and other Asian countries for thousands of years [1, 2]
A study has demonstrated that the therapeutic effects of moxibustion may be possibly based on the biological effects induced by thermal stimulation [7], which is closely related to the activation of thermoreceptors and nociceptive receptors, heat-sensitive immune cells (Langerhans cells and their major histocompatibility complex II), and heat shock proteins (HSP) at local acupoints [8]
We found that different moxibustion temperatures had variable effects on the mitochondrial respiratory chain complexes I–V activity and their key regulatory element proteins sirtuins 1 (SIRT1) and sirtuins 3 (SIRT3) expression. is may provide a basis for energy conversion from thermal stimulation to biological effect by moxibustion intervention
Summary
To study how thermal energy is converted after moxibustion at local skin from the view of mitochondrial respiratory chain and its key regulatory elements of sirtuins 1 (SIRT1) and sirtuins 3 (SIRT3). E activity of mitochondrial respiratory chain complexes I–V was detected by spectrophotometry. Moxibustion at 38°C triggered more significant increase of mitochondrial respiratory chain complexes I–V expression. The effect of mitochondrial respiratory chain complexes I–V activity on local skin of ST36 acupoint was more obvious at 30 min after moxibustion, while the expression of SIRT1 and SIRT3 protein was more significant at 72 h after moxibustion. Mitochondrial respiratory chain and its key regulatory element proteins SIRT1 and SIRT3 play important role in the initial process of thermal energy conversion stimulated by different moxibustion temperatures in local skin
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