Abstract
Cells of Fleischmann bakers' yeast were grown in standard nutrient broth and in broth to which cobalt, or cadmium, or thallium, had been added. The cells were fixed by glutaradehyde-permanganate and sectioned. Electron microscopy showed that ( a ) the endoplasmic reticulum was fixed well in cells grown in cobalt or cadmium, but the endoplasmic reticulum was not fixed in cells grown in normal or thallium broth; ( b ) the cristate mitochondria were normal in all cells except those grown in cadmium. No cristae were visible in the “cristate” mitochondria of cells grown in cadmium broth; ( c ) a large fraction of the cells recovered from cadmium broth were respiratory-deficient; ( d ) thallic oxide was present in the cristate mitochondria of cells recovered from thallium broth.
Published Version
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