Abstract
Sulfur dioxide (SO2) maintains a certain steady state balance in the body, high concentration SO2 will be harmful to human health. Seeking a suitable detection method to monitor sulfur dioxide in real time becomes an urgent requirement owing to the transient nature of sulfur dioxide in organisms. Here, a novel NIR ratiometric fluorescent probe for detection of SO2 was developed based on a conjugation of coumarin and indol salt with excellent water solubility. The probe Mito-CI displayed highly sensitive (69nM), fast response time (30s), large Stokes shift (174nm) and the NIR fluorescence emission wavelength (655nm). In the reversibility process of the SO3--probe Mito-CI system induced by HCHO in vitro was also detected. Besides, cell imaging showed that Mito-CI possesses mitochondria-targeted ability. Particularly, Mito-CI was proved to reversibly detect SO2/HCHO in living cells.
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