Missing Data Calculation Using the Antioxidant Activity in Selected Herbs

Donatella Bálint,Lorentz Jäntschi

doi:10.3390/sym11060779

Abstract

In this paper, a model has been developed that can estimate the composition of the phenol compounds, based on censored data and the total equivalent antioxidant capacity (TEAC) measured by three different methods. A contingency of 32 plants was analyzed: total phenolic content (TPC), caffeic acid, p-coumaric acid, ferulic acid, neochlorogenic acid and TEAC. They were measured by three different methods: ABTS (2,20-azinobis-(3-ethylbenzthiazoline- 6-sulfonic acid)), DPPH (1,1-diphenyl-2-picrylhydrazyl radical) and FRAP (ferric reducing/antioxidant power). Five values of caffeic-, thirteen of p-coumaric-, seven of ferulic-, and nineteen neochlorogenic acids were missing. Due to the complexity of the compounds, data mining and computational methods are required to determine the missing data. The method developed for independent variables was used to estimate the missing data. The contingency was filled with the calculated values obtained with all alternatives. The performance of each approach is shown in the estimation and/or prediction of the phenolic composition compared to the approaches used. The results indicated a strong correlation and mutual influence between the data analyzed.

Highlights

Plants contain a wide variety of free radical scavenging molecules such as phenolic compounds, nitrogen compounds, vitamins and terpenoids [1,2,3].These natural products [4] are used to provide proactive support to various physiological systems.They can cure or prevent diseases [5,6].Natural antioxidants can help preventing oxidation and regulate the immune function [7].Overproduction of free radicals can cause oxidative damage to biomolecules
The total antioxidant capacity expressed as total equivalent antioxidant capacity (TEAC) was estimated by the following methods: ABTS, DPPH and ferric reducing/antioxidant power (FRAP) [30]
The TEAC experimental values were excluded from further calculations because their χ2 were outliers

Summary

Introduction

Plants (fruits, vegetables, herbs, etc.) contain a wide variety of free radical scavenging molecules such as phenolic compounds (e.g., phenolic acids, flavonoids, quinones), nitrogen compounds (e.g., alkaloids, amines), vitamins and terpenoids [1,2,3].These natural products [4] are used to provide proactive support to various physiological systems.They can cure or prevent diseases (atherosclerosis, cancer, diabetes, degenerative diseases) [5,6].Natural antioxidants can help preventing oxidation and regulate the immune function [7].Overproduction of free radicals can cause oxidative damage to biomolecules. Plants (fruits, vegetables, herbs, etc.) contain a wide variety of free radical scavenging molecules such as phenolic compounds (e.g., phenolic acids, flavonoids, quinones), nitrogen compounds (e.g., alkaloids, amines), vitamins and terpenoids [1,2,3]. These natural products [4] are used to provide proactive support to various physiological systems. Overproduction of free radicals can cause oxidative damage to biomolecules. This can lead to many chronic and acute illnesses. Nowadays, examining the antioxidant capacity of plants has become an important subject [8]

Methods

Results

Conclusion