MiRNA‑885‑3p inhibits docetaxel chemoresistance in lung adenocarcinoma by downregulating AuroraA.

Abstract

AuroraA is a member of the mitotic serine/threonine kinase family. It is involved in key processes during mitosis and meiosis, and AuroraA upregulation is implicated in malignant transformation. In the present study, we revealed that AuroraA expression was significantly higher in docetaxel‑resistant lung adenocarcinoma (LAD) cells than in parental cells. Higher levels of AuroraA expression were significantly correlated with higher chemoresistance and proliferation in LAD cells, while silencing AuroraA promoted caspase‑3‑dependent cell apoptosis by downregulating NF‑κB and Bcl‑2 and upregulating Bax expression. In addition, an increased proportion of cells in the G2/Mphase and a decreased proportion of cells in the Sphase were observed due to the suppression of AuroraA. Furthermore, we identified that microRNA‑885‑3p (miR‑885‑3p) could target AuroraA directly. There was significantly lower miR‑885‑3p expression in docetaxel‑resistant LAD cells than in parental LAD cells. miR‑885‑3p could modulate the docetaxel response, cell proliferation and apoptosis in LAD cells invitro. Moreover, we found that AuroraA overexpression or miR‑885‑3p inhibition was associated with more aggressive behaviour in LAD cells. Thus, miR‑885‑3p/AuroraA may be involved in the chemoresistance of LAD cells, and assessing miR‑885‑3p/AuroraA expression may be a potential method for indicating chemosensitivity to docetaxel‑based chemotherapy.