Abstract
Background Glioblastoma (GBM) is an aggressive adult brain tumor that poses a huge threat to people's health. Previous studies have shown that microRNAs (miRNAs) are important regulators in the progression of GBM. However, the role of miR-448 in GBM remains largely unknown. Therefore, the regulatory mechanism of miR-448 in the development of GBM is elucidated in this study. Methods The protein and mRNA expressions of miR-448 and ROCK1 were measured by Western blot analysis and RT-qPCR. Cell proliferation, migration, and invasion were detected by CCK-8 assay and Transwell assay. The relationship between miR-448 and ROCK1 was probed by luciferase reporter assay. Results miR-448 expression was downregulated in GBM tissues and cells. And poor clinical outcomes of GBM patients were related to miR-448 downregulation. Functionally, overexpression of miR-448 restrained cell viability, migration, and invasion in GBM. Additionally, miR-448 reduced ROCK1 expression by binding to its 3′-UTR. Moreover, knockdown of ROCK1 inhibited the progression of GBM. Furthermore, overexpression of ROCK1 abolished the antitumor effect of miR-448 in GBM. Conclusion miR-448 restrained cell viability, invasion, and migration in GBM by inhibiting ROCK1 expression.
Highlights
Glioblastoma (GBM) is a grade IV glioma, which mainly occurs in middle-aged people aged 40-60 years
Primary normal human astrocytes (NHAs) and GBM cell lines A-172 MG, T98G were purchased from ATCC (Manassas, VA, USA)
We found that the expression of Rho-associated protein kinase 1 (ROCK1) was higher in A172MG and T98G cells than in NHAs cells
Summary
Glioblastoma (GBM) is an aggressive adult brain tumor that poses a huge threat to people’s health. The role of miR-448 in GBM remains largely unknown. The regulatory mechanism of miR-448 in the development of GBM is elucidated in this study. The protein and mRNA expressions of miR-448 and ROCK1 were measured by Western blot analysis and RT-qPCR. Migration, and invasion were detected by CCK-8 assay and Transwell assay. MiR-448 expression was downregulated in GBM tissues and cells. Poor clinical outcomes of GBM patients were related to miR-448 downregulation. Overexpression of miR-448 restrained cell viability, migration, and invasion in GBM. MiR-448 reduced ROCK1 expression by binding to its 3′-UTR. Knockdown of ROCK1 inhibited the progression of GBM. Overexpression of ROCK1 abolished the antitumor effect of miR-448 in GBM. MiR-448 restrained cell viability, invasion, and migration in GBM by inhibiting ROCK1 expression Conclusion. miR-448 restrained cell viability, invasion, and migration in GBM by inhibiting ROCK1 expression
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