MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development.

Min Chen,Min Chen,Min Chen,Min Chen,Shailendra Kumar Mishra,Xiaolan Fan,Yan Li,Mingwang Zhang,Huadong Yin,Qing Zhu,Mingyao Yang,Xiaoling Zhao,Deying Yang,Jian Gao,Diyan Li,Zhongxian Xu,Bo Zeng,Yan Wang,Shaolan Zhang,Qingyong Ni

doi:10.3389/fgene.2020.00570

Abstract

MicroRNA (miRNA) is known to be an important regulator of muscle growth and development. The regulation of microRNA on the skeletal muscle phenotype of animals is mainly achieved by regulating the proliferation and differentiation of myoblasts. In this study, we sequenced a total of 60 samples from 15 developing stages of the pectoral muscle and five other tissues at 300 days of Tibetan chicken. We characterized the expression patterns of miRNAs across muscle developmental stages, and found that the chicken growth and development stage was divided into early-embryonic and late-embryonic as well as postnatal stages. We identified 81 and 21 DE-miRNAs by comparing the miRNA profiles of pectoral muscle of three broad periods and different tissues, respectively; and 271 miRNAs showed time-course patterns. Their potential targets were predicted and used for functional enrichment to understand their regulatory functions. Significantly, GgmiRNA-454 is a time-dependent and tissue-differential expression miRNA. In order to elucidate the role of gga-miRNA-454 in the differentiation of myoblasts, we cultured chicken myoblasts in vitro. The results show that although gga-miRNA-454-3p initiates increase and thereafter decrease during the chicken myoblasts differentiation, it had no effect on primary myoblasts proliferation. Furthermore, we confirm that gga-miRNA-454 inhibits myoblast differentiation by targeting the myotube-associated protein SBF2.

Highlights

Skeletal muscle is a type of striated muscle tissue responsible for all voluntary movement in animals
We sequenced a total of 60 samples from 15 developing stages of the pectoral muscle and five other tissues at 300 days of Tibetan chicken
We characterized the expression patterns of miRNAs across muscle developmental stages, and found that the chicken growth and development stage was divided into early-embryonic, late embryo and postnatal stages

Summary

Introduction

Skeletal muscle is a type of striated muscle tissue responsible for all voluntary movement in animals. It accounts for half of the total body weight of the chicken. It is an important organization involved in regulation of animal metabolism, and strength (Pavlath and Horsley, 2003). Previous research on Tibetan chicken muscles involved physical. MiRNA Analysis of Chicken Muscle characteristics and processing properties (Qiu et al, 2011). Some assessed the adaptation of lowland chickens to highland from several aspects of liquid characteristics, blood gas and blood volume (León and Monge, 2004). Previous reports on Tibetan chickens have focused mainly on the physiological, biochemical and molecular mechanisms related to the adaptation of highaltitude environments (Monge and Leónvelarde, 1991; Xiao et al, 2005; Weber, 2007). There are few studies on the growth and development of Tibetan chickens, especially the regulation of miRNA on muscle growth and development of Tibetan chicken

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Discussion

Conclusion