Abstract
Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mechanism of gga-mir-133a-3p in the proliferation and differentiation of chicken myoblasts. Through the detection of cell proliferation activity, cell cycle progression and EdU, we found that gga-mir-133a-3p can significantly inhibit the proliferation of myoblasts. In the process of myogenic differentiation, gga-mir-133a-3p is up-regulated, while gga-mir-133a-3p can significantly promote the up-regulation of differentiation-related muscle-derived factors, indicating that gga-mir-133a-3p can promote the differentiation of myoblasts. Validation at the transcriptional level and protein level proved that gga-mir-133a-3p can inhibit the expression of PRRX1, and the dual-luciferase assay also showed their direct targeting relationship. Correspondingly, PRRX1 can significantly promote myoblast proliferation and inhibit myoblast differentiation. In our study, we confirmed that gga-mir-133a-3p participates in the regulation of proliferation and differentiation of myoblasts by targeting PRRX1.
Highlights
Skeletal muscle is the most important part of the body of the meat-producing animals, and its growth and development are closely related to the meat production
We revealed a role of gga-mir-133a-3p in the proliferation and differentiation of chicken myoblasts, and we found it is based on the function of regulating the proliferation and differentiation of myoblasts by inhibiting PRRX1 (Supplementary Figure S1)
Mir133 participated in myoblasts proliferation and differentiation by regulating the negatively modulates IGF-1R/PI3K/Akt signaling through repression of IGF-1R (Huang et al, 2011)
Summary
Skeletal muscle is the most important part of the body of the meat-producing animals, and its growth and development are closely related to the meat production. The coordinated regulation of myogenic renewal is a major part of skeletal muscle growth and development, including myoblast proliferation, differentiation, apoptosis, and fusion (Sartorelli and Fulco, 2004). The process of myoblast proliferation and differentiation is regulated by many signaling pathways and regulatory factors, including DNA, non-coding RNAs (ncRNAs), and peptides. Another study showed that mir-16-5p regulates p53 signaling pathways to affect myoblast proliferation apoptosis and differentiation by target to SESN1 (Cai et al, 2018). These results further identify the importance of microRNAs in the growth and development of chicken skeletal muscles
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
