MiRNA expression profiling of EV71 infected human neuroblastoma cells

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Objective To study the miRNA expression profiling in EV71 infected human neuroblastoma (SH-SY5Y) cells and to identify the possible effects of host miRNAs in the neurological pathogenesis of EV71 infection. Methods The EV71 infected SH-SY5Y cell model was established and the cells were collected after 48 h of infection. miRNA expression profiling in EV71 infected SHSY5Y cells was performed using Taqman Low-Density Array (TLDA) and was validated using real-time RT-PCR. The putative target genes for the 10 candidate miRNAs were predicted using Targetscan and miRanda online algorithms. The target genes were then analyzed by GO enrichment and KEGG pathway analysis. Results The EV71 infected cell model was successfully established. The TLDA results showed 215 up-regulated and 25 down-regulated miRNAs in the EV71 infected cells compared with controls. The expression levels of 3 down-regulated miRNAs (miR-10a*, miR-15b*and miR-195) and 7 up-regulated miRNAs (miR-10a, miR-342-5p, miR-483-5p, Let-7b, miR-99a, miR-140-5p and miR-21) were confirmed by RT-qPCR. The verification results of 10 candidate miRNAs were basically in agreement with the array results. The GO enrichment analysis showed that the target genes were enriched into the category of developmental process and regulation of signaling. KEGG pathway analysis suggested that these target genes were involved in many important pathways, including pathways in cancer, endocytosis, Wnt signaling pathway, melanogenesis, adherens junction and MAPK signaling pathway. Conclusions The miRNA expression profiling of SH-SY5Y cells changed after 48h infected with EV71. The 10 miRNAs played an important role in developmental process, regulation of signaling and apoptosis. Our finding may assist future researches on the pathogenesis mechanisms of EV71 infection. Key words: EV71; miRNA profiling; SH-SY5Y; Central nervous system