MiRNA-4665-3p Regulates Expression of PLD5 in Thyroid Cancer Patients and Predicts Death

Abstract

Thyroid cancer has become an increasingly common malignant tumor around the world, and its incidence is increasing year by year. In this study, mRNA microarray data of thyroid cancer patients from four periods were collected from the TCGA database. We performed a series of bioinformatics analyses on these mRNA expression profiles, including differential analysis, co-expression analysis, enrichment analysis, regulator prediction, and survival analysis. There were 13126, 10914, 13585, and 13241 differential genes in the four periods; 4822 differential genes were obtained by union and deduplication (p < 0.01). Weighted gene co-expression network analysis indicated a total of 21 functional disorder modules. In each module, PLD5, CHD4, ADGRA3, ITGA3, etc. were the key genes. Enrichment analysis showed that the dysfunctional module genes were mainly related to pre-replicative complex assembly, Cytokine–cytokine receptor interaction, and MAPK signaling pathway. We downloaded thyroid cancer-associated miRNA microarray data from the GEO database for differential analysis. Then, we crossed the predicted ncRNA with the differential miRNA to obtain thyroid cancer-associated regulatory factors. Finally, we found that miRNA-4665-3p regulates the core gene PLD5, and six regulators such as miRNA-3140-3p and miRNA-324-3p regulate the core gene CHD4. Survival analysis showed that both up-regulation of PLD5 expression and down-regulation of CHD4 expression accelerated patient death. According to the above analysis, we believe miRNA-4665-3p regulates the expression of PLD5 and affects the development of thyroid cancer. Its up-regulation promotes the death of patients.