Abstract
Aim: The purpose of our work was to evaluate the correlation between miRNA-122a and diagnosis and treatment of prostatic cancer. Methods : The 30 tumor and normal tissues were collected from prostatic cancer cases, evaluated miRNA-122a and Foxm1 by ISH, IHC and RT-PCR methods, and analyzing the correlation between miRNA-122a and Foxm1. In the cell experiment, Dividing DU-145 and PC-3 as 3 groups: NC groups, BL groups and miRNA groups. Using transwell and wound healing test to evaluate cell invasion and migration abilities. Using WB test to measure the relative proteins levels. Results: miRNA-122a and Foxm1 expressions of tumor tissues was significantly differences compared with adjacent normal tissues (P < 0.05y ). MiRNA-122a was negative correlation with Foxm1 in prostatic cancer tissues. The invasion cell number and wound healing rate of miRNA groups were significantly down-regulation compared with NC groups in DU-145 and PC-3 (P < 0.05). The relative proteins levels of miRNA groups were significantly differences compared with NC in DU-145 and PC-3 (P < 0.05). Conclusion : miRNA-122a might be a suppressor biomarker in the prostatic cancer.
Published Version
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