MiR-7 regulates intestinal trefoil factor and affects intestinal epithelial cell proliferation and migration

Abstract

Objective To investigate the regulation of miR-7 on intestinal trefoil factor (TFF3) and its effect on the proliferation and migration of intestinal epithelial cells. Methods miR-7 mimics and miR-7 inhibitor were transfected into LS174T cells respectively.The experiment was divided into 5 groups, including blank control group, miR-7 mimic negative control group, miR-7 mimic group, miR-7 inhibitor negative control group, miR-7 inhibitor group.MTT assay was used to detect cell proliferation.Cell scratch assay was used to detect the effect of miR-7 on cell migration.Western blot was used to detect the change of TFF3 protein in each group. Results Compared with the blank control group, the miR-7 mimic negative control group and the miR-7 inhibitor negative control group, the OD value of the miR-7 mimic group decreased significantly, the difference was statistically significant(P<0.05); the cell scratch interval increased, the cell migration rate decreased, the difference was statistically significant(P<0.05); and the TFF3 protein expression was accompanied(P<0.05). The OD value of the miR-7 inhibitor group significantly increased, the difference was statistically significant(P<0.05); the cell scratch gap decreased, the cell migration rate was enhanced, the difference was statistically significant(P<0.05); and the expression of TFF3 protein increased(P<0.05). Conclusion miR-7 can regulate the expression of TFF3 and further inhibit the proliferation and migration of LS174T cells. Key words: miR-7; Intestinal epithelial cells; Proliferation; Migration; Intestinal trefoil factor