Mechanism of miR-7 affecting migration ability of intestinal epithelial cells

Abstract

Objective To investigate the effect of miR-7 on the migration ability of intestinal epithelial cells and its related mechanisms. Methods LS174T cells were transfected with miR-7 mimic and miR-7 inhibitor respectively.The effect of miR-7 on cell migration ability was detected by transwell chamber.The changes of PI3K, p-Akt and Akt protein in each group were detected by Western Blot. Results Compared with the control group, miRNA mimic negative control group and miRNA inhibitor negative control group, the number of cells in the miR-7 mimic group through the tranwell chamber decreased, the cell migration rate decreased, and the expression of PI3K and p-Akt protein decreased at the same time, the difference was statistically significant(P<0.05). In the miRNA inhibitor group, the number of cells in the tranwell chamber increased, the cell migration rate increased, and the expression of PI3K and p-Akt protein was increased at the same time, the difference was statistically significant(P<0.05). Conclusion miR-7 can inhibit the migration of LS174T cells, which may be through the regulation of the PI3K/Akt signaling pathway. Key words: miR-7; Intestinal epithelial cell; Migration; Mechanism