Abstract
Objective MicroRNAs (miRNAs) have emerged as important regulators involved in malignant progression in some cancers. miR-328-3p has been found to function as a tumor suppressor in some tumors including non-small cell lung cancer (NSCLC). The article investigated the functional role of miR-328-3p in NSCLC. Methods The expression of miR-328-3p was determined by qRT-PCR in both NSCLC tissue and cells. The cell proliferation and invasion were assessed by CCK-8, cell colony formation and trans-well assays. Luciferase reporter assay demonstrated that PINK1 was a target of miR-328-3p. Western blot assay detected the cyclinB1, cyclinD1 and PINK1protein expression. Results In the study, our results showed that miR-328-3p was significantly down-regulated in NSCLC tissues and cell lines (P=0.0312). Furthermore, upregulation of miR-328-3p significantly suppressed the NSCLC cell proliferation, colony formation, and cell invasion capacities. Moreover, upregulation of miR-328-3p inhibited CyclinB1 and CyclinD1 expression in both A549 and H1229 cells. Luciferase activity assay results demonstrated that PINK1 was a direct target of miR-328-3p, and miR-328-3p inhibited the cell proliferation by regulating the mRNA and protein levels of PINK1 in NSCLC cells. Conclusion These data suggest that miR-328-3p plays a tumor suppressing role and may be a potential therapeutic target for NSCLC patients. Key words: Non-small cell lung cancer; miR-328-3p; PINK1; Cell proliferation
Published Version
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