MiR-30a-3p/IGF2BP1 affect the proliferation and migration of lung adenocarcinoma cells

Abstract

Background: IGF2BP1 is a carcinomatous fetal protein associated with proliferation, migration and invasion of tumor cells. The different roles of miR-30a-3p as a tumor suppressor or an oncogene depend on the target genes it regulates. Methods: Downloading the relevant data of lung adenocarcinoma from the TCGA database for bioinformatics analysis to determine the key gene IGF2BP1 and its miRNA that affect the progression of lung adenocarcinoma, and using qPCR to verify their expression and correlation in lung adenocarcinoma. Gene enrichment analysis of IGF2BP1 related pathways, and the function of miR-30a-3p/IGF2BP1 axis in lung adenoma was verified by CCK-8 detection, MTT assay, double luciferase gene reporting assay, cell cloning assay and wound healing assay. Results: As predicted, IGF2BP1 was highly expressed in lung adenocarcinoma tissues, affecting TNM stage and survival status. In addition, IGF2BP1 was negatively correlated with the expression of miR-30a-3p, and the low expression of miR-30a-3p had a poor survival period. Luciferase result confirmed the existence of a targeting relationship between miR-30a-3p and IGF2BP1. Gene enrichment analysis showed that the high expression of IGF2BP1 was correlated with the proliferation of lung adenocarcinoma cells. The experimental results of this study showed that cell viability, proliferation and migration of cells were decreased in the si-IGF2BP1 group and miR-30a-3p-mimics group. In the wound healing assay, compared with the mimics group, the migration ability of cells in the mimics+oe-IGF2BP1 group was improved, which was consistent with the results in the control group. Conclusion: miR-30a-3p can affect the activity of lung adenocarcinoma cells and promote cell proliferation via regulating IGF2BP1. The miR-30a-3p/IGF2BP