MiR-23b inhibits proliferation and migration of triple negative breast cancer cells

Abstract

Objective To investigate the effect and mechanism of miR-23b on the proliferation and migration of triple negative breast cancer (TNBC)cells. Methods Real time polymerase chain reaction (PCR) was used to detect the expression of miR-23b in triple negative breast cancer tissues. MDA-MB-231/miR-23b, BT549/miR-23b cell lines are constructed. Proliferation assay, scaling healing experiment and Transwell migration assay were used to detect the effect of miR-23b on the proliferation and migration of triple negative breast cancer cells. Dual-luciferase reporter gene assay was employed to examine the interactions between miR-23b and forkhead box C2 (FOXC2). Real time PCR and Western blot were performed to detect the effect of miR-23b on the expression of FOXC2. Results The expression level of miR-23b in triple negative breast cancer tissues was significantly less than that in adjacent normal tissues. miR-23b could reduced the proliferation and migration of triple negative breast cancer cells. Dual-luciferase assay confirmed that miR-23b could regulate the expression and activity of FOXC2. The expression of FOXC2 in mRNA and protein level was inhibited by miR-23b. Conclusions miR-23b can inhibit the expression of FOXC2 and affect the proliferation and migration of triple negative breast cancer cells. Key words: MicroRNAs; Breast neoplasms; Cell proliferation; Cell migration assays; In vitro