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Abstract
IntroductionLong noncoding RNAs (lncRNAs) have been associated with many types of cancers, but their molecular mechanisms in lung squamous cell carcinoma (LUSC) have not been fully studied. Therefore, the current study investigated the regulation role of microRNA-205 host gene (MIR205HG) in LUSC and recognized the target genes managed by this lncRNA.MethodsMIR205HG expression was assessed by the quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The effects of silenced MIR205HG on cell biological behaviors were detected by colony formation assay, transwell assay, flow cytometry analysis and western blot analysis. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay were utilized to proof the binding relationship between miR-299-3p and MIR205HG/mitogen-activated protein kinase kinase kinase 2 (MAP 3 K2).ResultsThe expression levels of MIR205HG in LUSC tissues and cell lines were obviously up-regulated. Down-regulation of MIR205HG expression remarkably reduced cell proliferation, migration and epithelial-to-mesenchymal transition (EMT) progression, whereas promoted cell apoptosis. MIR205HG could bind with miR-299-3p and down-regulation of MIR205HG elevated miR-299-3p expression. MAP 3 K2 acted as the target gene of miR-299-3p and was up-regulated by MIR205HG overexpression. Overexpressing MAP 3 K2 could counteract the effects of down-regulating MIR205HG on LUSC progression to some degree.ConclusionMIR205HG acts as a competing endogenous RNA (ceRNA) to expedite cell proliferation and progression via targeting miR-299-3p in LUSC.
Highlights
Long noncoding RNAs have been associated with many types of cancers, but their molecular mechanisms in lung squamous cell carcinoma (LUSC) have not been fully studied
The results depicted that MIR205HG was significantly up-regulated in LUSC tissues compared with the adjacent normal tissues (Fig. 1a)
QRT-PCR detected that MIR205HG expression was relatively high in LUSC cell lines (NCI-H520, SKMES-1 and NCI-H1703) compared with the normal control (Fig. 1b)
Summary
Long noncoding RNAs (lncRNAs) have been associated with many types of cancers, but their molecular mechanisms in lung squamous cell carcinoma (LUSC) have not been fully studied. The current study investigated the regulation role of microRNA-205 host gene (MIR205HG) in LUSC and recognized the target genes managed by this lncRNA. Plenty of studies have revealed that long noncoding RNAs (lncRNAs) may serve as crucial cis- or transregulators in multiple biological behaviors [2, 7]. MicroRNA-205 host gene (MIR205HG) has been proofed to accelerate cell growth in Head and Neck Squamous Cell Carcinoma (HNSCC) by down-regulating miR-590-3p [8]. Studies on the function of MIR205HG in the progression of LUSC are still blank, so its biological functions are needed to be featured
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