MiR-96-5p alleviates inflammatory responses by targeting NAMPT and regulating the NF-κB pathway in neonatal sepsis.

Xueli Chen,Li Dai,Ying Chen,Na Wang

doi:10.1042/bsr20201267

Abstract

Neonatal septicemia is a serious infectious disease in the neonatal period. MicroRNAs (miRNAs) have been reported to participate in the inflammatory responses in neonatal sepsis. The aim of the present study was to explore the effects and molecular mechanism of miR-96-5p on regulating the inflammatory responses in neonatal sepsis. MiR-96-5p was low expressed while nicotinamide phosphoribosyltransferase (NAMPT) was high expressed in the serum of neonatal septicemia patients. The expression of miR-96-5p was decreased in LPS-induced inflammatory responses. Besides, miR-95-5p relieved LPS-induced inflammatory responses in RAW264.7 cells. NAMPT was demonstrated as a potential target of miR-96-5p, and knockdown of NAMPT reduced inflammatory in RAW264.7 cells stimulated with LPS. Moreover, overexpression of NAMPT reversed the effects of miR-96-5p on LPS-induced inflammatory responses. In addition, miR-96-5p inhibited nuclear factor (NF)-κB signaling pathway in RAW264.7 cells stimulated with LPS. MiR-96-5p alleviated inflammatory responses via targeting NAMPT and inhibiting NF-κB pathway in neonatal sepsis.

Highlights

Neonatal sepsis is a common disease in newborn infants and has high morbidity and mortality [1]
The expression levels of miR-96-5p and nicotinamide phosphoribosyltransferase (NAMPT) were first detected in neonatal sepsis and control serum by reverse transcription-quantitative PCR (qRT-PCR), and the result shown that miR-96-5p level was significantly decreased in the serum of neonatal sepsis compared with the control (Figure 1A)
The NAMPT protein level was detected using Western blot, and the result showed that NAMPT expression was increased in neonatal sepsis (Figure 1D,E), which was consistent with the qRT-PCR result

Summary

Introduction

Neonatal sepsis is a common disease in newborn infants and has high morbidity and mortality [1] It is the third leading cause of neonatal death and accounts for approximately 25% of neonatal mortality [2]. MiRNAs are a kind of short noncoding RNAs containing 19–22 nucleotides It suppresses mRNA expression by combining with the 3 -untranslated region (UTR) of target genes and resulting degradation or transcriptional inhibition of target mRNA [5,6]. MiR-96-5p regulates spinal cord injury through the NF-κB pathway [11]. It is unclear whether miR-96-5p plays a role in neonatal septicemia

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