Abstract

 
 
 
 Purpose: To explore miRNA-875-5p and epidermal growth factor receptor (EGFR) activities in tissues or cells from cervical cancer, and their underlying molecular mechanisms.
 Methods: Tissues were obtained from cervical cancer patients and their miR-875-5p expression was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Caski or HeLa cells were transfected with miR-875-5p mimics or miR-875-5p inhibitor to assess the effect of miR-875- 5p expression on cell viability, cell cycle, migration, and invasion using Cell Counting Kit-8 (CCK-8), flow cytometry, wound healing, and Transwell assays. Potential target genes of miR-875-5p were predicted and verified using a dual luciferase reporter assay. In addition, EGFR expression was evaluated by western blot.
 Results: MicroRNA-875-5p was expressed at low levels in cervical cancer tissues and was related to FIGO stage, lymph node metastasis, pathological grade, vascular involvement, and deep stromal invasion in patients with cervical cancer. MicroRNA-875-5p overexpression inhibited cell viability, migration, and invasion, and caused G0/G1 phase block of Caski and HeLa cells. Moreover, EGFR was the target gene of miR-875-5p and was negatively regulated by miR-875-5p. Reductions in cell viability, migration, invasion, and the number of G0/G1-phase cells were inhibited by EGFR overexpression.
 Conclusion: MiR-875-5p suppresses cervical cancer cell growth and metastasis by negatively regulating EGFR. Therefore, miR-875-5p can potentially be exploited for the management of cervical cancer.
 
 
 
Highlights
Cervical cancer is second to breast cancer as the most common gynecologic malignancy [1]
This study further investigated the co-regulatory effects of miR-875-5p and epidermal growth factor receptor (EGFR) on cervical cancer cells
Further results from this study showed that miR875-5p suppressed cell viability and metastasis and promoted cell cycle block, confirming that miR-875-5p is a tumor suppressor gene for cervical cancer
Summary
Cervical cancer is second to breast cancer as the most common gynecologic malignancy [1]. The incidence of cervical cancer has been increasing and tending toward younger ages over the past decade [2]. The course of cervical cancer generally involves cervical hyperplasia, early invasive carcinoma, and cervical cancer [2]. More than 50% of patients have progressed to invasive carcinoma by the time they are diagnosed, and at this stage invasion and metastasis greatly reduce their survival. Patients with cervical cancer usually have a poor prognosis and lower survival rate, and the underlying pathological mechanisms of. The biological functions of miR-8755p and its potential molecular mechanisms in cervical cancer cells were investigated by in vitro cell experiments, with the aim of seeking new therapeutic avenues for cervical cancer
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