MiR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat

Ying-Hui Ling,Li-Na Xu,Meng-Hua Sui,Yong Liu,Wen-Yong Li,Fu-Gui Fang,Hao Wu,Ming-Xing Chu,Kang-Yan Wang,Qi Zheng

doi:10.1038/s41598-018-22262-4

Abstract

This study found that miR-27 is expressed in muscle and regulates muscle proliferation and differentiation. We explored the function and regulatory mechanism of miR-27b in goat muscle proliferation and differentiation. Compared with the Boer goat, higher expression of miR-27b was observed in all of the collected muscle tissues of Anhuai goat, excluding the kidney, whereas the opposite expression pattern was observed for Pax3, which showed lower expression in Anhuai goat. Expression of miR-27b decreased gradually during the proliferation of skeletal muscle satellite cells in Anhuai goat and increased during differentiation; however, the expression pattern of Pax3 was opposite. The regulatory activity of miR-27b demonstrated that miR-27b inhibited the proliferation of skeletal muscle satellite cells, but promoted their differentiation. Moreover, function research demonstrated that Pax3 negatively regulated myogenic differentiation of goat skeletal muscle satellite cells, but accelerated their proliferation. The results of a dual-luciferase reporter analysis showed that miR-27b directly targeted the 3’-untranslated regions of Pax3 mRNA, and western blot and immunofluorescence staining analyses showed that miR-27b inhibited expression of the Pax3 protein. In goats, miR-27b can regulate myogenic proliferation and differentiation by targeting Pax3.

Highlights

The goat is an important economic animal in China, and the consumption of mutton is increasing each year
Myogenesis is regulated by a number of transcription factors and several noncoding RNAs, which act at specific steps, including the commitment of progenitor cells to myogenic lineage and myoblast proliferation, differentiation, and fusion11,12. miRNAs comprise a class of non-coding RNAs that suppress mRNA translation or induce degradation, and it plays a critical role in many processes, such as cell proliferation and differentiation, among others
Family, miR-27b plays an important role in muscle development. miR-27b can inhibit skeletal muscle satellite cell proliferation but promote its differentiation[16]

Summary

Introduction

The goat is an important economic animal in China, and the consumption of mutton is increasing each year. Myogenesis is regulated by a number of transcription factors and several noncoding RNAs, which act at specific steps, including the commitment of progenitor (satellite) cells to myogenic lineage and myoblast proliferation, differentiation, and fusion. MiR-27b can regulate Pax[3] protein levels, and this downregulation ensures rapid and robust entry into the myogenic differentiation programme in mice[16]. These studies were all discovered in mouse, human or bovine, and few experiments describe the potential role of miRNAs in goat skeletal muscle satellite cells. We analysed the expression patterns of miR-27b and Pax[3] in different muscle tissues of goat and studied the potential of miR-27b as a regulator of Pax[3] in Anhuai goat. miR-27b targets the 3′-UTR of Pax[3] and affects the post-transcriptional expression of Pax[3], with consequences for the onset of skeletal muscle differentiation

Methods

Results

Conclusion