Abstract
Akirin2, a novel nuclear factor, plays an important role in myogenesis. To investigate the role of Akirin2 in proliferation and differentiation of porcine skeletal muscle satellite cells, Akirin2 overexpression and Akirin2 silence technologies were employed. Our results showed that overexpression of Akirin2 markedly enhanced the proliferation and differentiation of porcine skeletal muscle satellite cells, whereas silencing of Akirin2 got the opposite results. Furthermore, our results showed that Akirin2 affected proliferation and differentiation of porcine skeletal muscle satellite cells through extracellular-signal regulated kinase-1/2 (ERK1/2) and NFATc1 signaling pathways. These results indicate that Akirin2 can effectively promote skeletal muscle satellite cells proliferation and differentiation, acting through ERK1/2- and NFATc1-dependent mechanisms.
Highlights
Akirin[2], a novel nuclear factor, plays an important role in myogenesis
There is some evidence that nuclear factor of activated T cells c1 (NFATc1) cooperates with extracellular signal-regulated kinase 1/2 (ERK1/2) signaling in the induction of cell proliferation, apoptosis and differentiation[12,13,14]
We found that Akirin[2] mRNA was upregulated during porcine skeletal muscle satellite cells differentiation, which was similar to myogenin mRNA (Fig. 2)
Summary
Akirin[2], a novel nuclear factor, plays an important role in myogenesis. To investigate the role of Akirin[2] in proliferation and differentiation of porcine skeletal muscle satellite cells, Akirin[2] overexpression and Akirin[2] silence technologies were employed. Our results showed that overexpression of Akirin[2] markedly enhanced the proliferation and differentiation of porcine skeletal muscle satellite cells, whereas silencing of Akirin[2] got the opposite results. Our results showed that Akirin[2] affected proliferation and differentiation of porcine skeletal muscle satellite cells through extracellularsignal regulated kinase-1/2 (ERK1/2) and NFATc1 signaling pathways. Myogenesis is a multistep process, which involves skeletal muscle satellite cell activation, proliferation, differentiation and formation of multinucleated myofibers[1,2]. Akirin[2] overexpression and Akirin[2] silence were employed to study the effects and the underlying mechanism of Akirin[2] on the proliferation and differentiation of porcine skeletal muscle satellite cells
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