Abstract
Objective miR-19a-3p is widely increased in several cancers and can be used as an oncogenic factor in these cancers. However, the molecular mechanism of miR-19a-3p in bladder urothelial carcinoma (BLCA) is still open. So, the study was aimed at exploring the mechanism of miR-19a-3p in BLCA cells. Methods Bioinformatics analysis was employed to find the differential miRNAs and mRNAs, and the target miRNA and mRNA were determined. Real-time quantitative PCR was used to evaluate miR-19a-3p and THBS1 levels in human urethral epithelial cells and BLCA cells. Western blot was carried out to assay protein expression of THBS1 in human urethral epithelial cells and BLCA cells. Behaviors of BLCA cells were detected through cellular functional assays. Dual-luciferase gene assay was conducted to validate the binding of miR-19a-3p and THBS1. Results miR-19a-3p was increased in BLCA cells, while THBS1 was less expressed in BLCA cells. The miR-19a-3p functions as an oncogene in BLCA. THBS1 was a target of miR-19a-3p, and it could reverse the promotion of miR-19a-3p on cell malignant behaviors in BLCA. Conclusion miR-19a-3p facilitates cell progression in BLCA via binding THBS1, which may be an underlying therapeutic target for BLCA treatment.
Highlights
According to cancer statistics in China, the mortality and morbidity of bladder cancer rank first among all malignant tumors in the urinary system, which seriously reduces the quality of life of human beings [1]
Bladder cancer can be divided into the bladder epithelial tumor and bladder nonepithelial tumor, of which the bladder epithelial tumor accounts for 95%-98% [2,3,4]
Surgery is dominant for bladder urothelial carcinoma (BLCA) management, but a high recurrence rate with frequent local infiltration and distant metastasis of BLCA leads to unsatisfactory treatment effects on patients with advanced BLCA [5, 6]
Summary
According to cancer statistics in China, the mortality and morbidity of bladder cancer rank first among all malignant tumors in the urinary system, which seriously reduces the quality of life of human beings [1]. Based on the regulatory effect of miRNA on the progression of BLCA, numerous miRNAs are promising molecular therapeutic targets of BLCA, such as miR133b [14], miR-429 [15], and miR-331-3p [16]. It is still unknown whether miR-19a-3p is dysregulated in Computational and Mathematical Methods in Medicine. We confirmed in this research the dysregulation of miR19a-3p in BLCA and observed the impact of miR-19a-3p on BLCA progression through biological function experiments to identify whether miR-19a-3p can regulate the progression of BLCA. The molecular mechanism of miR-19a-3p was probed in BLCA so as to lay the groundwork for miR19a-3p as a molecular target for BLCA patients
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