Abstract
BackgroundThe human miR-17-92 polycistron is the first reported and most well-studied onco-miRNA with a cluster of seven miRNAs. miR-17-5p, a member of the miR-17-92 family, plays an important role in tumor cell proliferation, apoptosis, migration and invasion. However, few studies have shown the role of miR-17-5p in the cell cycle of head and neck squamous cell carcinoma (HNSCC).MethodsRT-qPCR was used to detect miR-17-5p expression levels in 64 HNSCC tissues and 5 cell lines. The relationship between the expression of miR-17-5p in the tissues and the clinical characteristics of the patients was analyzed. HNSCC cells were transfected with an miR-17-5p mimic or inhibitor to evaluate cell cycle distribution by flow cytometry. Cell cycle distribution of cells transfected with target gene was evaluated using flow cytometry. Dual-luciferase reporter assay was used to detect the regulatory effect of miR-17-5p on target gene expression.ResultsIn the present study, we found that miR-17-5p expression in HNSCC tissues and cell lines was remarkably increased, and miR-17-5p is related to recurrence in HNSCC patients. Silencing miR-17-5p blocked HNSCC cells in G2/M phase, whereas its overexpression propelled cell cycle progression. More importantly, we verified that miR-17-5p negatively regulated CCNG2 mRNA and protein expression by directly targeting its 3’UTR.ConclusionThese findings suggest that miR-17-5p might act as a tumor promoter and prognostic factor for recurrence in HNSCC patients.
Highlights
Head and neck squamous cell carcinoma (HNSCC) was the seventh most common cancer worldwide in 2018 (890,000 new cases and 450,000 deaths), accounting for 3% of all cancers and just over 1.5% of all cancer deaths in the United States [1]
Results miR-17-5p is upregulated in head and neck squamous cell carcinoma (HNSCC) tissues and cell lines and is related to HNSCC recurrence To investigate the role of miR-17-5p in HNSCC development, we first ascertained the level of miR-17-5p in HNSCC tissues. qRT-PCR result showed that miR-17-5p level was significantly upregulated in HNSCC tissues (n = 64) compared with that in vocal cord polys tissues (n = 18) (Fig. 1A)
We found that HNSCC patients with higher levels of miR-17-5p had a higher likelihood of recurrence compared with patients who had a lower level of miR-17-5p (p = 0.0023; Fig. 1C)
Summary
Head and neck squamous cell carcinoma (HNSCC) was the seventh most common cancer worldwide in 2018 (890,000 new cases and 450,000 deaths), accounting for 3% of all cancers and just over 1.5% of all cancer deaths in the United States [1]. MiR-17-5p has been implicated in cancer development, including proliferation, apoptosis, migration, and invasion. Wang et al found that dysregulation of miR-17-5p/PIK3R1 axis participated in laryngeal squamous cell carcinoma (LSCC) cell proliferation and apoptosis by inhibiting the activation of the PI3K/AKT signaling pathway [5]. Few studies have shown the role of miR-17-5p in the cell cycle of HNSCC. The human miR-17-92 polycistron is the first reported and most well-studied onco-miRNA with a cluster of seven miRNAs. miR-17-5p, a member of the miR-17-92 family, plays an important role in tumor cell proliferation, apoptosis, migration and invasion. Few studies have shown the role of miR-17-5p in the cell cycle of head and neck squamous cell carcinoma (HNSCC)
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