Abstract
Renal cell carcinoma (RCC) is a common cancer, and extensive research suggests that microRNA may play an important role in the progression of RCC. The emphasis of this article was to reveal the function and mechanism of microRNA-1293(miR-1293) in the development of RCC tumors. First, the authors carried out bioinformatics analysis. The differential expression of miR-1293 in RCC tumor and normal cells was analyzed using the data from The Cancer Genome Atlas database, and Kaplan-Meier survival analysis was carried out to test the survival rate. Subsequently, the miR-1293 expression in RCC cell lines was examined by quantitative real-time PCR. Then Cell counting kit-8 and Transwell assays were executed to detect the function of miR-1293 in RCC. Bioinformatics prediction, western blotting, and dual-luciferase reporter assay were set to check the target gene of miR-1293. Finally, they conducted rescue experiments to verify whether the regulation of miR-1293 on the biological function of RCC cells was achieved by regulating hydrocyanic oxidase 2 (HAO2). Bioinformatics results showed that miR-1293 was highly expressed in RCC, and the miR-1293 high-expression group showed a lower survival rate than the miR-1293 low-expression group, which suggested that the high expression of miR-1293 was related to unfavorable prognosis in RCC. Subsequent assays evidenced that upregulation of miR-1293 expression significantly increased the cell viability and promoted cell migration and invasion in RCC. Silencing miR-1293 expression showed opposite results. Furthermore, HAO2 was confirmed to be a direct target gene of miR-1293 by dual-luciferase reporter assay, and miR-1293 negatively regulated the expression of HAO2. Moreover, rescue experiments evidenced that miR-1293 reduced the cell viability, invasion, and migration of RCC by regulating HAO2. In sum, miR-1293 can regulate the viability, invasion, and migration of RCC tumor cells by targeting HAO2, suggesting that miR-1293 can be used as a new biomarker for clinical treatment of RCC.
Highlights
Renal cell carcinoma (RCC) is considered to be the most common primary renal malignancy, accounting for 90%–95% of all renal cancer cases, with a mortality rate of 25%.1,2 In recent years, surgical resection is the most recommended treatment for RCC.[3]
The results indicated that in the miR-1293 inhibitor plus si-hydrocyanic oxidase 2 (HAO2) group, the viability of 786-O cells and the number of 786-O cells migrating and invading into the lower chamber increased significantly compared with the miR-1293 inhibitor group ( p < 0.01, Fig. 7A, C, and D), whereas ACHN cells co-transfected with miR-1293 mimic and pcDNA3.1-HAO2 significantly reduced cell viability, migration, and invasion compared with ACHN cells transfected with miR-1293 mimic ( p < 0.01, Fig. 7B, E, and F)
The authors found that miR-1293 was highly expressed in RCC, and high expression was associated with unfavorable prognosis
Summary
Renal cell carcinoma (RCC) is considered to be the most common primary renal malignancy, accounting for 90%–95% of all renal cancer cases, with a mortality rate of 25%.1,2 In recent years, surgical resection is the most recommended treatment for RCC.[3]. Of biological behaviors such as proliferation, apoptosis, invasion, and migration of cancer cells, playing a carcinogenic or anticancer role.[8,9] Extensive research has confirmed that miRNAs can be involved in the regulation of the process of RCC. On the basis of existing literature, the authors carried out studies in an effort to quest whether miR-1293 plays a role in regulating cell viability, migration, and invasion in RCC, and to further explore its mechanism, hoping to supply a potential biomarker for the therapy of RCC
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