MiR-127-3p Inhibits Breast Cancer Cell Behaviors via Targeting Benzodiazepine Receptor-Associated Protein 1 (BZRAP1)

Abstract

microRNAs are key regulators of cell proliferation, apoptosis, and anti-cancer immune response. This study intends to evaluate miR-127-3p’s role in breast cancer cells (BC). After transfection, miR-156 and BZRAP1 expression was assessed by qRT-PCR and Western blotting along with analysis of cell proliferation and apoptosis by MTT and flow cytometry. Finally, an in vivo tumor model was established to verify miR-127-3p’s in vivo effect. Transfection of si-BZRAP1/miR-127-3p into MCF-7 cells reduced BZRAP1 expression, inhibited cell proliferation and promoted apoptosis. miR-127-3p is confirmed to target BZRAP1 and exerts tumor suppressor activity by inhibiting BZRAP1. miR-127-3p inhibited BC cell growth and promote apoptosis by targeting BZRAP1, indicating that it is expected to be a target for the treatment of BC. The significance of this study is to confirm that miR-127-3p may participate in tumor progression via BZRAP1, and may become a potential target for treating tumor. Further analysis of the pathogenesis of breast cancer and detection of miR-127-3p/BZRAP1 in BC has important application value in the treatment.